Cell Culture:Article Title: Deep and fast label-free Dynamic Organellar Mapping.
Article Snippet: Antibodies.Antibodies.. The following antibodies were used in this study: mouse (IgG2b) anti-GALNT2 1:200 for IF (BioLegend Cat# 682302, RRID:AB_2566611), rabbit anti-GLG1 1:200 for IF (Sigma Aldrich Cat# SAB1303679), mouse (IgG1) anti-GOLIM4 1:1000 for IF (Enzo Life Sciences Cat# ALX-804-603-C100, RRID:AB_2051552), mouse anti-LC3B 1:400 for IF (MBL International Cat# M152-3, RRID:AB_1279144), rabbit anti-SDF4 1:400 for IF (Sigma Aldrich Cat# HPA011249, RRID:AB_2668468), sheep anti-TGN46 (TGOLN2) 1:200 for IF (Bio-Rad Cat# AHP500, RRID:AB_324049) and rabbit anti-TM9SF2 1:200 for IF (Abcam Cat# ab271123).. Fluorescently labelled secondary antibodies were purchased fromThermoFisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID: AB_141607), Alexa Fluor 555-labelled goat anti-rabbit IgG (Cat#A32732, RRID:AB_2633281), Alexa Fluor 568-labelled donkey anti-rabbit IgG (Cat# A10042, RRID:AB_2534017), Alexa Fluor 555-labelled donkey anti-sheep IgG (Cat# A-21436, RRID:AB_2535857) and Alexa Fluor 680- labelled donkey anti-sheep IgG (Cat# A-21102, RRID:AB_2535755).Fluorescently labelled secondary antibodies were purchased fromThermoFisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID:..
Article Title: Deep and fast label-free Dynamic Organellar Mapping
Article Snippet: The following antibodies were used in this study: mouse (IgG2b) anti-GALNT2 1:200 for IF (BioLegend Cat# 682302, RRID:AB_2566611), rabbit anti-GLG1 1:200 for IF (Sigma Aldrich Cat# SAB1303679), mouse (IgG1) anti-GOLIM4 1:1000 for IF (Enzo Life Sciences Cat# ALX-804-603-C100, RRID:AB_2051552), mouse anti-LC3B 1:400 for IF (MBL International Cat# M152-3, RRID:AB_1279144), rabbit anti-SDF4 1:400 for IF (Sigma Aldrich Cat# HPA011249, RRID:AB_2668468), sheep anti-TGN46 (TGOLN2) 1:200 for IF (Bio-Rad Cat# AHP500, RRID:AB_324049) and rabbit anti-TM9SF2 1:200 for IF (Abcam Cat# ab271123).. Fluorescently labelled secondary antibodies were purchased from Thermo Fisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID: AB_141607), Alexa Fluor 555-labelled goat anti-rabbit IgG (Cat# A32732, RRID:AB_2633281), Alexa Fluor 568-labelled donkey anti-rabbit IgG (Cat# A10042, RRID:AB_2534017), Alexa Fluor 555-labelled donkey anti-sheep IgG (Cat# A-21436, RRID:AB_2535857) and Alexa Fluor 680-labelled donkey anti-sheep IgG (Cat# A-21102, RRID:AB_2535755).Fluorescently labelled secondary antibodies were purchased from Thermo Fisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat#..
Immunofluorescence:Article Title: Deep and fast label-free Dynamic Organellar Mapping.
Article Snippet: Antibodies.Antibodies.. The following antibodies were used in this study: mouse (IgG2b) anti-GALNT2 1:200 for IF (BioLegend Cat# 682302, RRID:AB_2566611), rabbit anti-GLG1 1:200 for IF (Sigma Aldrich Cat# SAB1303679), mouse (IgG1) anti-GOLIM4 1:1000 for IF (Enzo Life Sciences Cat# ALX-804-603-C100, RRID:AB_2051552), mouse anti-LC3B 1:400 for IF (MBL International Cat# M152-3, RRID:AB_1279144), rabbit anti-SDF4 1:400 for IF (Sigma Aldrich Cat# HPA011249, RRID:AB_2668468), sheep anti-TGN46 (TGOLN2) 1:200 for IF (Bio-Rad Cat# AHP500, RRID:AB_324049) and rabbit anti-TM9SF2 1:200 for IF (Abcam Cat# ab271123).. Fluorescently labelled secondary antibodies were purchased fromThermoFisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID: AB_141607), Alexa Fluor 555-labelled goat anti-rabbit IgG (Cat#A32732, RRID:AB_2633281), Alexa Fluor 568-labelled donkey anti-rabbit IgG (Cat# A10042, RRID:AB_2534017), Alexa Fluor 555-labelled donkey anti-sheep IgG (Cat# A-21436, RRID:AB_2535857) and Alexa Fluor 680- labelled donkey anti-sheep IgG (Cat# A-21102, RRID:AB_2535755).Fluorescently labelled secondary antibodies were purchased fromThermoFisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID:..
Article Title: Deep and fast label-free Dynamic Organellar Mapping
Article Snippet: The following antibodies were used in this study: mouse (IgG2b) anti-GALNT2 1:200 for IF (BioLegend Cat# 682302, RRID:AB_2566611), rabbit anti-GLG1 1:200 for IF (Sigma Aldrich Cat# SAB1303679), mouse (IgG1) anti-GOLIM4 1:1000 for IF (Enzo Life Sciences Cat# ALX-804-603-C100, RRID:AB_2051552), mouse anti-LC3B 1:400 for IF (MBL International Cat# M152-3, RRID:AB_1279144), rabbit anti-SDF4 1:400 for IF (Sigma Aldrich Cat# HPA011249, RRID:AB_2668468), sheep anti-TGN46 (TGOLN2) 1:200 for IF (Bio-Rad Cat# AHP500, RRID:AB_324049) and rabbit anti-TM9SF2 1:200 for IF (Abcam Cat# ab271123).. Fluorescently labelled secondary antibodies were purchased from Thermo Fisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID: AB_141607), Alexa Fluor 555-labelled goat anti-rabbit IgG (Cat# A32732, RRID:AB_2633281), Alexa Fluor 568-labelled donkey anti-rabbit IgG (Cat# A10042, RRID:AB_2534017), Alexa Fluor 555-labelled donkey anti-sheep IgG (Cat# A-21436, RRID:AB_2535857) and Alexa Fluor 680-labelled donkey anti-sheep IgG (Cat# A-21102, RRID:AB_2535755).Fluorescently labelled secondary antibodies were purchased from Thermo Fisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat#..
Imaging:Article Title: Deep and fast label-free Dynamic Organellar Mapping.
Article Snippet: Antibodies.Antibodies.. The following antibodies were used in this study: mouse (IgG2b) anti-GALNT2 1:200 for IF (BioLegend Cat# 682302, RRID:AB_2566611), rabbit anti-GLG1 1:200 for IF (Sigma Aldrich Cat# SAB1303679), mouse (IgG1) anti-GOLIM4 1:1000 for IF (Enzo Life Sciences Cat# ALX-804-603-C100, RRID:AB_2051552), mouse anti-LC3B 1:400 for IF (MBL International Cat# M152-3, RRID:AB_1279144), rabbit anti-SDF4 1:400 for IF (Sigma Aldrich Cat# HPA011249, RRID:AB_2668468), sheep anti-TGN46 (TGOLN2) 1:200 for IF (Bio-Rad Cat# AHP500, RRID:AB_324049) and rabbit anti-TM9SF2 1:200 for IF (Abcam Cat# ab271123).. Fluorescently labelled secondary antibodies were purchased fromThermoFisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID: AB_141607), Alexa Fluor 555-labelled goat anti-rabbit IgG (Cat#A32732, RRID:AB_2633281), Alexa Fluor 568-labelled donkey anti-rabbit IgG (Cat# A10042, RRID:AB_2534017), Alexa Fluor 555-labelled donkey anti-sheep IgG (Cat# A-21436, RRID:AB_2535857) and Alexa Fluor 680- labelled donkey anti-sheep IgG (Cat# A-21102, RRID:AB_2535755).Fluorescently labelled secondary antibodies were purchased fromThermoFisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID:..
Article Title: Deep and fast label-free Dynamic Organellar Mapping
Article Snippet: The following antibodies were used in this study: mouse (IgG2b) anti-GALNT2 1:200 for IF (BioLegend Cat# 682302, RRID:AB_2566611), rabbit anti-GLG1 1:200 for IF (Sigma Aldrich Cat# SAB1303679), mouse (IgG1) anti-GOLIM4 1:1000 for IF (Enzo Life Sciences Cat# ALX-804-603-C100, RRID:AB_2051552), mouse anti-LC3B 1:400 for IF (MBL International Cat# M152-3, RRID:AB_1279144), rabbit anti-SDF4 1:400 for IF (Sigma Aldrich Cat# HPA011249, RRID:AB_2668468), sheep anti-TGN46 (TGOLN2) 1:200 for IF (Bio-Rad Cat# AHP500, RRID:AB_324049) and rabbit anti-TM9SF2 1:200 for IF (Abcam Cat# ab271123).. Fluorescently labelled secondary antibodies were purchased from Thermo Fisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID: AB_141607), Alexa Fluor 555-labelled goat anti-rabbit IgG (Cat# A32732, RRID:AB_2633281), Alexa Fluor 568-labelled donkey anti-rabbit IgG (Cat# A10042, RRID:AB_2534017), Alexa Fluor 555-labelled donkey anti-sheep IgG (Cat# A-21436, RRID:AB_2535857) and Alexa Fluor 680-labelled donkey anti-sheep IgG (Cat# A-21102, RRID:AB_2535755).Fluorescently labelled secondary antibodies were purchased from Thermo Fisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat#..
Control:Article Title: Deep and fast label-free Dynamic Organellar Mapping.
Article Snippet: Antibodies.Antibodies.. The following antibodies were used in this study: mouse (IgG2b) anti-GALNT2 1:200 for IF (BioLegend Cat# 682302, RRID:AB_2566611), rabbit anti-GLG1 1:200 for IF (Sigma Aldrich Cat# SAB1303679), mouse (IgG1) anti-GOLIM4 1:1000 for IF (Enzo Life Sciences Cat# ALX-804-603-C100, RRID:AB_2051552), mouse anti-LC3B 1:400 for IF (MBL International Cat# M152-3, RRID:AB_1279144), rabbit anti-SDF4 1:400 for IF (Sigma Aldrich Cat# HPA011249, RRID:AB_2668468), sheep anti-TGN46 (TGOLN2) 1:200 for IF (Bio-Rad Cat# AHP500, RRID:AB_324049) and rabbit anti-TM9SF2 1:200 for IF (Abcam Cat# ab271123).. Fluorescently labelled secondary antibodies were purchased fromThermoFisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID: AB_141607), Alexa Fluor 555-labelled goat anti-rabbit IgG (Cat#A32732, RRID:AB_2633281), Alexa Fluor 568-labelled donkey anti-rabbit IgG (Cat# A10042, RRID:AB_2534017), Alexa Fluor 555-labelled donkey anti-sheep IgG (Cat# A-21436, RRID:AB_2535857) and Alexa Fluor 680- labelled donkey anti-sheep IgG (Cat# A-21102, RRID:AB_2535755).Fluorescently labelled secondary antibodies were purchased fromThermoFisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID:..
Article Title: Deep and fast label-free Dynamic Organellar Mapping
Article Snippet: The following antibodies were used in this study: mouse (IgG2b) anti-GALNT2 1:200 for IF (BioLegend Cat# 682302, RRID:AB_2566611), rabbit anti-GLG1 1:200 for IF (Sigma Aldrich Cat# SAB1303679), mouse (IgG1) anti-GOLIM4 1:1000 for IF (Enzo Life Sciences Cat# ALX-804-603-C100, RRID:AB_2051552), mouse anti-LC3B 1:400 for IF (MBL International Cat# M152-3, RRID:AB_1279144), rabbit anti-SDF4 1:400 for IF (Sigma Aldrich Cat# HPA011249, RRID:AB_2668468), sheep anti-TGN46 (TGOLN2) 1:200 for IF (Bio-Rad Cat# AHP500, RRID:AB_324049) and rabbit anti-TM9SF2 1:200 for IF (Abcam Cat# ab271123).. Fluorescently labelled secondary antibodies were purchased from Thermo Fisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat# A-21202, RRID: AB_141607), Alexa Fluor 555-labelled goat anti-rabbit IgG (Cat# A32732, RRID:AB_2633281), Alexa Fluor 568-labelled donkey anti-rabbit IgG (Cat# A10042, RRID:AB_2534017), Alexa Fluor 555-labelled donkey anti-sheep IgG (Cat# A-21436, RRID:AB_2535857) and Alexa Fluor 680-labelled donkey anti-sheep IgG (Cat# A-21102, RRID:AB_2535755).Fluorescently labelled secondary antibodies were purchased from Thermo Fisher Scientific and used at 1:500 for IF: Alexa Fluor 488-labelled donkey anti-mouse IgG (Cat#..
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